BIOFECTOR is an integrated project that develops alternative fertilisation strategies by the use of various bio-effectors (BEs, plant growth promoting microorganisms and natural extraction products). BEs stimulate root growth, solubilise and mineralise sparingly available nutrients, or protect plants from abiotic and biotic stresses. Novel BEs will be isolated, characterized and applied in strategic combination with alternative fertilisation strategies that include organic and low-input farming, use of waste recycling fertilizers, and fertiliser-placement technologies.Bio-effectors addressed comprise fungal strains of Trichoderma, Penicillium and Sebacinales, as well as bacterial strains of Bacillus and Pseudomonades with well-characterized root growth promoting and nutrient solubilising potential. Natural extraction products of seaweed, compost and plant extracts, as well as their purified active compounds are also tested in various combinations. Maize, wheat and tomato are chosen as representative crops. Laboratory and European-wide field experiments assure product adaptation to divers geo-climatic conditions. Viable alternatives to the conventional practice of mineral fertilisation are developed, towards environmental friendly agricultural practice with reduced agrochemical input.