ESTEEM2 is an integrated infrastructure of electron microscopy facilities providing access for the academic and industrial research community in the physical sciences to some of the most powerful characterization techniques available at the nanoscale. Transnational access to ESTEEM2 centres is obtained through a transparent, simple peer review process based on merit and scientific priorities. Service to users is supported by a networking programme which addresses key issues such as specimen preparation, data interpretation through theory and simulation, and standardization of protocols and methodologies. A series of schools and workshops provide training in innovative methods in electron microscopy and a forum for discussing emerging (cutting-edge) techniques. Directed research programmes focus on the further development of electron diffraction, imaging and spectroscopy and the advancement of 3D methods and time resolved experiments. In all, ESTEEM2 establishes a strategic leadership in electron microscopy to guide future developments and promote electron microscopy to the wider research community at large.