This project will demonstrate on large scale the sustainable production of bio-fuels based on low-cost microalgae cultures. The full chain of processes from algal ponds to biomass separation, processing for oil and other chemicals extraction, and downstream biofuel production, as well as the use in vehicles, will be implemented on a 10 ha site.Depending on the methodology chosen during the research phase of the project, and the sustainability analysis, the most suitable site for the objectives will be selected, among a number of selected locations in the South of Spain (Chiclana, Almeria, Sevilla, Arcos, Canary Islands…) Wastewater influent and nutrients will be re-used to stimulate algae growth. The extracted oils will be processed at an existing biodiesel plant (capacity 6000 t/yr of used oils), designed by a consortium partner, which can be converted at reasonable cost.The specified algae yield of 100 t/ha/yr will be obtained by integrating and upscaling innovative systems to double algal yields. With a net oil content of 20 %, enough biodiesel yearly to run close to 200 cars is expected. The residual algae will be digested together with the wastewater solids in order to produce biogas and CO2. The biogas will be purified and compressed to serve as vehicle fuel for a number of cars equivalent or superior to the ones fuelled by liquids.To reach the enhanced algal yield, additional CO2 will be obtained by the thermal transformation of external biomass (i.e, sludge from a wastewater treatment plant located in the area), together with internal biomass( digestate from residual algae and wastewater solids), to generate flue-gas as heating source for drying the biomass previous to Combustion. The options to transform the excess thermal energy generated into electricity to power the systems will be analysed for financial and technical viability.As some key process elements have not yet been proven on industrial scale, the technical risks will be limited by an initial prototype phase. During these initial 2 -3 years, the whole chain will be built and operated with each unit in its maximum size – for instance, two ponds systems of 1000 to 2000 m2 linked to the respective harvesting, processing and digestion.The prototype as well as the future full-scale facility will be investigated for environmental and social impacts in order to maximize sustainability. These results and specific diffusion efforts will allow extrapolation to other sites.