In this project, we aim to develop nanotechnology-based systems for detection of pathogenic bacteria in several aqueous media mainly in environmental water based on suspension arrays together with a portable custom-designed detection system. The six objectives (six work packages) are as follows: (i) Nanostructured sensors surfaces, in which electron-beam, photo-, soft and dip-pen lithographies will be applied in the fabrication of functionalized low-cost (disposable) SERS substrates, specially designed for simultaneus Raman and Fluorescence enhancement; (ii) nanosorbents and their mixtures as suspension arrays against the target bacteria for capturing units in which firstly magnetically loaded nanoparticles will be produced and labelled/barcoaded with fluorosence dies and/or quantum dots and then bacteriophages, monoclonal antibodies, and/or aptamers will be immobilized onto these nanoparticles as bioligands to recognize and capture the target bacteria specifically; their mixtures will form the suspension arrays; (iii) target bacteria and bacteriophages; the target bacteria (as water pollutants), are Escherichia coli, Enterococcus species and Bacteroides ovatus; bacteriophages which will recognize these bacteria will be selected/produced as bioligands; (iv) aptamers; they will be selected/modified/produced; (v) sensors/array systems; a portable “Raman Spectrometer/Fluorescence Detector System” will be designed/produced that will be used together with the nanostructured sensors platforms, and finally (vi) validation; the materials and systems developed will be validated. This is a multidisciplinary and technological project and therefore brings together experts from different disciplines both from academia and industry. Basicly, the knowledge that will be developed/ accumulated at lab scale during the project will be transfered to the industrial partners for prototype productions, and then these will bring back to the academical institutions for validation tests.